{"id":23417,"date":"2020-06-10T19:12:59","date_gmt":"2020-06-10T19:12:59","guid":{"rendered":"https:\/\/usoceandiscovery.org\/?page_id=23417"},"modified":"2022-08-13T08:20:24","modified_gmt":"2022-08-13T08:20:24","slug":"kyle-metcalfe","status":"publish","type":"page","link":"https:\/\/usoceandiscovery.org\/kyle-metcalfe\/","title":{"rendered":"2019 Schlanger Fellow – Kyle Metcalfe"},"content":{"rendered":"<div class=\"fusion-fullwidth fullwidth-box fusion-builder-row-1 fusion-flex-container nonhundred-percent-fullwidth non-hundred-percent-height-scrolling\" style=\"background-color: rgba(255,255,255,0);background-position: center center;background-repeat: no-repeat;border-width: 0px 0px 0px 0px;border-color:var(--awb-color3);border-style:solid;\" ><div class=\"fusion-builder-row fusion-row fusion-flex-align-items-flex-start\" style=\"max-width:1456px;margin-left: calc(-4% \/ 2 );margin-right: calc(-4% \/ 2 );\"><div class=\"fusion-layout-column fusion_builder_column fusion-builder-column-0 fusion_builder_column_1_3 1_3 fusion-flex-column\"><div class=\"fusion-column-wrapper fusion-flex-justify-content-flex-start fusion-content-layout-column\" style=\"background-position:left top;background-repeat:no-repeat;-webkit-background-size:cover;-moz-background-size:cover;-o-background-size:cover;background-size:cover;padding: 2% 0px 0px 0px;\"><div style=\"text-align:center;\"><span class=\" fusion-imageframe imageframe-none imageframe-1 hover-type-none\"><a class=\"fusion-no-lightbox\" href=\"\/wp-content\/uploads\/2019\/03\/metcalfe_headshot-copy-300x300.png\" target=\"_self\" aria-label=\"metcalfe_headshot copy\"><img decoding=\"async\" width=\"300\" height=\"300\" src=\"https:\/\/usoceandiscovery.org\/wp-content\/uploads\/2019\/03\/metcalfe_headshot-copy-300x300.png\" alt class=\"img-responsive wp-image-22828\" srcset=\"https:\/\/usoceandiscovery.org\/wp-content\/uploads\/2019\/03\/metcalfe_headshot-copy-150x150.png 150w, https:\/\/usoceandiscovery.org\/wp-content\/uploads\/2019\/03\/metcalfe_headshot-copy-300x300.png 300w, https:\/\/usoceandiscovery.org\/wp-content\/uploads\/2019\/03\/metcalfe_headshot-copy-500x500.png 500w, https:\/\/usoceandiscovery.org\/wp-content\/uploads\/2019\/03\/metcalfe_headshot-copy-570x570.png 570w, https:\/\/usoceandiscovery.org\/wp-content\/uploads\/2019\/03\/metcalfe_headshot-copy-700x701.png 700w, https:\/\/usoceandiscovery.org\/wp-content\/uploads\/2019\/03\/metcalfe_headshot-copy-768x770.png 768w, https:\/\/usoceandiscovery.org\/wp-content\/uploads\/2019\/03\/metcalfe_headshot-copy.png 976w\" sizes=\"(max-width: 300px) 100vw, 300px\" \/><\/a><\/span><\/div><style type=\"text\/css\">@media only screen and (max-width:1024px) {.fusion-title.fusion-title-1{margin-top:10px!important; margin-right:0px!important;margin-bottom:15px!important;margin-left:0px!important;}}@media only screen and (max-width:640px) {.fusion-title.fusion-title-1{margin-top:10px!important; margin-right:0px!important;margin-bottom:10px!important; margin-left:0px!important;}}<\/style><div class=\"fusion-title title fusion-title-1 fusion-sep-none fusion-title-center fusion-title-text fusion-title-size-two\" style=\"margin-top:10px;margin-right:0px;margin-bottom:15px;margin-left:0px;\"><h2 class=\"title-heading-center fusion-responsive-typography-calculated\" style=\"margin:0;--fontSize:28;line-height:var(--awb-typography1-line-height);\">KYLE METCALFE<\/h2><\/div><div class=\"fusion-text fusion-text-1\" style=\"text-align:center;margin-top:0%;\"><p><b>California Institute of Technology<\/b><\/p>\n<\/div><\/div><style type=\"text\/css\">.fusion-body .fusion-builder-column-0{width:33.333333333333% !important;margin-top : 0px;margin-bottom : 20px;}.fusion-builder-column-0 > .fusion-column-wrapper {padding-top : 2% !important;padding-right : 0px !important;margin-right : 5.76%;padding-bottom : 0px !important;padding-left : 0px !important;margin-left : 5.76%;}@media only screen and (max-width:1024px) {.fusion-body .fusion-builder-column-0{width:33.333333333333% !important;order : 0;}.fusion-builder-column-0 > .fusion-column-wrapper {margin-right : 5.76%;margin-left : 5.76%;}}@media only screen and (max-width:640px) {.fusion-body .fusion-builder-column-0{width:100% !important;order : 0;}.fusion-builder-column-0 > .fusion-column-wrapper {margin-right : 1.92%;margin-left : 1.92%;}}<\/style><\/div><div class=\"fusion-layout-column fusion_builder_column fusion-builder-column-1 fusion_builder_column_2_3 2_3 fusion-flex-column\"><div class=\"fusion-column-wrapper fusion-flex-justify-content-flex-start fusion-content-layout-column\" style=\"background-position:left top;background-repeat:no-repeat;-webkit-background-size:cover;-moz-background-size:cover;-o-background-size:cover;background-size:cover;padding: 0px 0px 0px 0px;\"><div class=\"fusion-text fusion-text-2\"><p><b><em>Mineral-Associated Microbial Ecology of the Deep Subsurface, Nankai Trough, Japan<\/em><\/b><\/p>\n<p><strong>Abstract<\/strong><\/p>\n<p>Minerals host the marine subsurface biosphere, but it remains unknown if specific minerals present in marine sediments play a role in facilitating microbial survival in the deep biosphere. Conductive minerals have been shown to stimulate the metabolic activity of microorganisms sampled from the subsurface, suggesting subsurface microorganisms may associate with these phases\u00a0<em>in situ<\/em>. I hypothesize that biosynthetically-active microorganisms in the deep biosphere associate with conductive minerals. I propose to test this hypothesis by applying mineral separation techniques to incubations of sediment from IODP Expedition 370 followed by downstream microbial community analysis via iTag sequencing corroborated by fluorescence\u00a0<em>in situ\u00a0<\/em>hybridization (FISH) and evaluation of biosynthetic activity employing bioorthogonal noncanonical amino acid tagging (BONCAT).<\/p>\n<p><strong>Biography<\/strong><\/p>\n<p>My interest in geobiology and environmental microbiology was first sparked during my undergraduate thesis with Professor Robert Gaines at Pomona College. Through this project, in which I examined dissimilatory reduction of ferric iron bound in clay minerals, I learned to appreciate the dazzling diversity of the microbial world and its profound impact on global biogeochemistry. I sought to further explore the interplay between microbiology and geochemistry through my PhD work with Dr. Victoria Orphan at Caltech, where I\u2019ve applied novel mineral separation techniques to seafloor sediments to parse the microbial ecology of sediments at unprecedented resolution. In my project funded by the Schlanger Fellowship, I will apply these novel techniques to sediments retrieved from the marine deep subsurface on IODP Expedition 370 with the aim of revealing new insight into microbe-mineral and microbe-microbe interactions in the deep biosphere.<\/p>\n<\/div><\/div><style type=\"text\/css\">.fusion-body .fusion-builder-column-1{width:66.666666666667% !important;margin-top : 0px;margin-bottom : 20px;}.fusion-builder-column-1 > .fusion-column-wrapper {padding-top : 0px !important;padding-right : 0px !important;margin-right : 0px;padding-bottom : 0px !important;padding-left : 0px !important;margin-left : 0px;}@media only screen and (max-width:1024px) {.fusion-body .fusion-builder-column-1{width:66.666666666667% !important;order : 0;}.fusion-builder-column-1 > .fusion-column-wrapper {margin-right : 0px;margin-left : 0px;}}@media only screen and (max-width:640px) {.fusion-body .fusion-builder-column-1{width:100% !important;order : 0;}.fusion-builder-column-1 > .fusion-column-wrapper {margin-right : 1.92%;margin-left : 1.92%;}}<\/style><\/div><\/div><style type=\"text\/css\">.fusion-body .fusion-flex-container.fusion-builder-row-1{ padding-top : 0px;margin-top : 0px;padding-right : 0px;padding-bottom : 0px;margin-bottom : 0px;padding-left : 0px;}<\/style><\/div>\n","protected":false},"excerpt":{"rendered":"","protected":false},"author":2,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"100-width.php","meta":{"_exactmetrics_skip_tracking":false,"_exactmetrics_sitenote_active":false,"_exactmetrics_sitenote_note":"","_exactmetrics_sitenote_category":0,"jetpack_post_was_ever_published":false,"footnotes":""},"class_list":["post-23417","page","type-page","status-publish","hentry"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v24.6 - 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